Anti-Double Stranded DNA Antibody (Anti-dsDNA) - Human

by USCNK
Rs. 20,540.00
SKU

Catalog No :RS01Ab0001

Organism- Human

Clonality :Polyclonal

Host :Rabbit

Concentration :200ug/ml

Application :WB, ICC, IHC-P, IHC-F, ELISA

Immunoglobulin Type : IgG

Purification :  Affinity Chromatography

Conjugate : No Conjugate

IMMUNOGEN INFORMATION
  • DsDNA (double stranded deoxyribonucleic acid) is a molecule that encodes the genetic instructions used in the development and functioning of all known living organisms and many viruses.
  • The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, A,T,G,C. These are linked together by 3',5'-phosphodiester bridges.
  • The two strands of DNA run in opposite directions to each other. Anti-dsDNA has been shown to be useful, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan, containing a giant mitochondrion.
  • The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis.
ANTIBODY SPECIFICITY
  • The antibody is a rabbit polyclonal antibody raised against Anti-dsDNA.
  • It has been selected for its ability to recognize Anti-dsDNA in immunohistochemical staining and western blotting.
APPLICATIONS
  • Western blotting: 1:100-400
  • Immunocytochemistry in formalin fixed cells: 1:100-500
  • Immunohistochemistry in formalin fixed frozen section: 1:100-500
  • Immunohistochemistry in paraffin section: 1:50-200
  • Enzyme-linked Immunosorbent Assay: 1:100-200
  • Optimal working dilutions must be determined by end user.
CONTENTS: Form & Buffer:
  • Supplied as solution form in PBS,
  • pH7.4,
  • containing 0.02% NaN3,
  • 50% glycerol.
STORAGE
  • Store at 4oC for frequent use.
  • Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity.
  • Avoid repeated freeze-thaw cycles

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