CHAI Green qPCR Master Mix

Rs. 22,200.00
SKU R02201S

Make: CHAI

Catalog No: R02201S


Chai Green™ Master Mix + Hot Start 2X is a highly optimized ready-to-use solution for Real-Time PCR. The mix combines Chai's aptamer-based hot start technology with Chai Green dye, resulting in exceptional quantitative PCR and High-Resolution Melt (HRM) performance. This mix is validated for products between 100 bp and 5 kb.


  • Multiplex up to four Real-Time PCR reactions with no effect on Cq’s
  • Extreme thermal stability: withstands three months at 25 °C with no impact on efficiency
  • Overcomes inhibition in crude extracts and environmental samples
  • Universal mix runs fast protocols, amplifies GC-rich targets, and incorporates aptamer-based hot start and dUTP carry-over prevention systems


Concentration 2X
Shipping Conditions Ice
Storage Temperature -20 °C  Protect from light
Exonuclease Activity 5' → 3'
Hot Start Yes
Recommended Reaction Size 10 – 50 µL



Component Amount
Taq polymerase 50 U/mL
Aptamer 20 nM
KCl 100 mM
MgCl2 6 mM
TrisCl pH 8.6 20 mM
Glycerol 10%
Trehalose 200 mM
BSA 0.4 mg/mL
Detergents 0.26%
dNTPs (each) 600 µM
Chai Green

10 µM



Amplification of Lambda Phage DNA Using Chai Green Master Mix + Hot Start 2X

Lambda Phage Standard Curve Amplified With Chai Green PCR Master Mix + Hot Start 2X

Chai Green Fluorescence Compared With SYBR Green I

Download Product Manual

Safety Data Sheet (SDS)

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