AFB1(Aflatoxin B1) ELISA Kit

Rs. 28,000.00

Catalog number:E-TO-E008

Test principle:

This kit uses Indirect-Competitive-ELISA as the method. It can detect AflatoxinB1 (AFB1) in samples, such as grain, peanut, formula feed, etc. This kit is composed of ELISA Microtiter plate HRP conjugate, antibody, standard, and other supplementary reagents. The microplate provided in this kit has been pre-coated with AFB1. During the reaction, AFB1 in the samples or standard competes with AFB1 on the solid phase supporter for sites of AFB1 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microplate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of AFB1. The concentration of AFB1 in the samples can be calculated by comparing the OD of the samples to the standard curve

Technical indicator:

Sensitivity: 0.03 ppb (ng/mL)

Reaction mode: 25℃, 30 min~ 15 min

Sample type: Grain,Formula feed,Edible oil,Peanut,Sauce/Wheat/Barley feed,Beer,Wine/Soy sauce/Vinegar

Detection limit: 

  • Grain---0.15 ppb, Formula feed---0.3 ppb, Edible oil/Peanut---0.6 ppb,
  •     Sauce/Wheat/Barley feed---0.3 ppb, Beer----0.3 ppb, 
  •     Wine/Soy sauce/Vinegar---0.15 ppb

Cross-reactivity: Aflatoxin B1 (AFB1) 100%

Sample recovery rate: 

  • Grain/Formula feed---85±15%, Peanut---82±15%, Edible oil---85±15%,
  •     Sauce/Wheat/Barley feed---83%±15%, Beer---84%±15%,
  •     Wine/Soy sauce/Vinegar---87%±15%

Kits components



ELISA Microtiter plate

96 wells

Standard Liquid

1mL each

(0 ppb, 0.03 ppb, 0.06 ppb, 0.12 ppb, 0.24 ppb, 0.48 ppb)

High Concentrated Standard (100 ppb)

1 mL

HRP Conjugate

5.5 mL

Antibody Working Solution

5.5 mL

Substrate Reagent A

6 mL

Substrate Reagent B

6 mL

Stop Solution

6 mL

20×Concentrated Wash Buffer

40 mL

Plate Sealer

3 pieces

Sealed Bag

1 piece


1 copy


Storage and valid period

Storage: Store at 2-8℃. Avoid freeze / thaw cycles.

Valid Period: 1 year, expiration plate date is on the packing box.



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