Anti-AGO2 Antibody

Rs. 45,619.00
SKU stj27819

General Information

Product name Anti-AGO2 Antibody
Short Description Rabbit polyclonal to AGO2
Description This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
Applications WB,IHC
Dilution range WB 1:500 - 1:1000
IHC 1:50 - 1:200
Protein Name Anti-AGO2 Antibody
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF2C2 (NP_036286.2).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 97.2 kDa
Database Links
Alternative Names AGO2Protein argonaute-2 antibodyArgonaute2 antibodyhAgo2AGO2 antibodyEIF2C2
Function Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC), The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA), These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing, The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target, Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2, Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity, May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E, May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit, The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur, In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR), Can also up-regulate the translation of specific mRNAs under certain growth conditions, Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation, Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Cellular Localization Cytoplasm, P-body.
Tissue Specificity
Swiss-Prot Key

 

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