Anti-AKT1S1 Antibody

Rs. 45,619.00
SKU stj27994

General Information

Product name Anti-AKT1S1 Antibody
Short Description Rabbit polyclonal to AKT1S1
Description
Applications WB,IHC
Dilution range WB 1:500 - 1:2000
IHC 1:50 - 1:200
Protein Name Anti-AKT1S1 Antibody
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 152-276 of human AKT1S1 (NP_115751.3).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 27.4 kDa
Database Links
Alternative Names AKT1S1Proline-rich AKT1 substrate 1 antibody40 kDa proline-rich AKT substrateAKT1S1 antibodyPRAS40
Function Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals, mTORC1 is activated in response to growth factors or amino acids, Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1, Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases, Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis, mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E), mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation, Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins, Inhibits RHEB-GTP-dependent mTORC1 activation, Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms, May also play a role in nerve growth factor-mediated neuroprotection.
Cellular Localization Cytoplasm, cytosol
Tissue Specificity Widely expressed with highest levels of expression in liver and heart, expressed at higher levels in cancer cell lines (e,g, a-549 and hela) than in normal cell lines (e,g, hek293).
Swiss-Prot Key

 

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