Anti-APEX1 Antibody

Rs. 45,619.00
SKU stj116180

General Information

Product name Anti-APEX1 Antibody
Short Description Rabbit polyclonal to APEX1
Description Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
Applications WB,IF,IP
Dilution range WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200
Protein Name DNA-(apurinic or apyrimidinic sitelyase lyasemitochondrial]
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 35.6 kDa
Database Links
Alternative Names APEX1DNA-(apurinic or apyrimidinic site antibodylyase lyase antibodymitochondrial]APEX1 antibodyAPE antibodyAPE1 antibodyAPEX antibodyAPX antibodyHAP1 antibodyREF1
Function Multifunctional protein that plays a central role in the cellular response to oxidative stress, The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors, Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents, Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends, Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules, Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER, Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks, May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation, Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB, Plays a role in the protection from granzymes-mediated cellular repair leading to cell death, Also involved in the DNA cleavage step of class switch recombination (CSR), On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR, Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs), Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression, Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance, Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism, Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD), In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression, Associates, together with YBX1, on the MDR1 promoter, Together with NPM1, associates with rRNA, Binds DNA and RNA.
Cellular Localization Nucleus, Nucleus, nucleolus, Nucleus speckle, Endoplasmic reticulum, Cytoplasm, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial: Mitochondrion.
Tissue Specificity
Swiss-Prot Key

 

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