Anti-BCL6 Antibody

Rs. 45,619.00
SKU stj29253

General Information

Product name Anti-BCL6 Antibody
Short Description Rabbit polyclonal to BCL6
Description The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.
Applications WB,IF
Dilution range WB 1:500 - 1:2000
IF 1:50 - 1:100
Protein Name Anti-BCL6 Antibody
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human BCL6 (NP_001697.2).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 78.8 kDa
Database Links
Alternative Names BCL6B-cell lymphoma 6 protein antibodyBCL-6 antibodyB-cell lymphoma 5 protein antibodyBCL-5 antibodyProtein LAZ-3 antibodyZinc finger and BTB domain-containing protein 27 antibodyZinc finger protein 51BCL6 antibodyBCL5 antibodyLAZ3 antibodyZBTB27 antibodyZNF51
Function Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions, Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes, Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors, In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155, An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response, In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells, Also required for the establishment and maintenance of immunological memory for both T- and B-cells, Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2, In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners, Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.
Cellular Localization Nucleus
Tissue Specificity Expressed in germinal center t- and b-cells and in primary immature dendritic cells.
Swiss-Prot Key

 

You recently viewed

Clear recently viewed