Anti-BRCA1 antibody

Rs. 50,211.00
SKU stj91882

General Information

Product name Anti-BRCA1 antibody
Short Description
Description Rabbit polyclonal to BRCA1.
Applications IHC,ELISA
Dilution range IHC 1:100-1:300ELISA 1:20000
Protein Name Anti-BRCA1 antibody
Immunogen Synthesized peptide derived from human BRCA1 around the non-phosphorylation site of S988.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 180 kDa
Database Links
Alternative Names Breast cancer type 1 susceptibility protein antibody
RING finger protein 53 antibody
RING-type E3 ubiquitin transferase BRCA1 antibody
RNF53 antibody
Function E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator .
Cellular Localization Nucleus Chromosome Cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs). recruitment to DNA damage sites is mediated by ABRAXAS1 and the BRCA1-A complex . Translocated to the cytoplasm during UV-induced apoptosis . Isoform 3: Cytoplasm.. Isoform 5: Cytoplasm
Tissue Specificity Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
Swiss-Prot Key

 

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