Anti-CASP8 Antibody

Rs. 45,619.00
SKU stj113797

General Information

Product name Anti-CASP8 Antibody
Short Description Rabbit polyclonal to CASP8
Description This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Applications WB,IF
Dilution range WB 1:500 - 1:1000
IF 1:50 - 1:100
Protein Name Caspase-8CASP-8
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-479 of human CASP8 (NP_001073593.1).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 55.4 kDa
Database Links
Alternative Names CASP8Caspase-8 antibodyCASP-8CASP8 antibodyMCH5
Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death, Binding to the adapter molecule FADD recruits it to either receptor, The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation, The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases, Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC, Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10, May participate in the GZMB apoptotic pathways, Cleaves ADPRT, Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC, Likely target for the cowpox virus CRMA death inhibitory protein, Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Cellular Localization Cytoplasm
Tissue Specificity Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues, highest expression in peripheral blood leukocytes, spleen, thymus and liver, barely detectable in brain, testis and skeletal muscle
Swiss-Prot Key

 

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