General Information
| Product name | Anti-Cdk7 antibody |
|---|---|
| Short Description | |
| Description | Rabbit polyclonal to Cdk7. |
| Applications | WB,IHC,ELISA |
| Dilution range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000 |
| Protein Name | Anti-Cdk7 antibody |
| Immunogen | Synthesized peptide derived from human Cdk7 around the non-phosphorylation site of T170. |
| Storage Instruction | Store at -20°C, and avoid repeat freeze-thaw cycles. |
| Type of Usage | For Research Use Only (RUO). |
Product Properties
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Reactivity | Mouse |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Isotype | IgG |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Target
| Gene ID | |
|---|---|
| Gene Symbol | |
| Molecular Weight | 40 kDa |
| Database Links | |
| Alternative Names | Cyclin-dependent kinase 7 antibody 39 kDa protein kinase antibody p39 Mo15 antibody CDK-activating kinase 1 antibody Cell division protein kinase 7 antibody Serine/threonine-protein kinase 1 antibody TFIIH basal transcription factor complex kinase subunit antibody CAK antibody CAK1 antibody CDKN7 antibody MO15 antibody STK1 antibody |
| Function | Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition. |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Colocalizes with PRKCI in the cytoplasm and nucleus. Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides. |
| Tissue Specificity | Ubiquitous. |
| Swiss-Prot Key |