Anti-CHEK1 Antibody

Rs. 68,577.00
SKU stj71518

General Information

Product name Anti-CHEK1 Antibody
Short Description Goat polyclonal to CHEK1
Applications ELISA, WB
Dilution range
Protein Name Anti-CHEK1 Antibody
Immunogen internal region
Storage Instruction
Type of Usage For Research Use Only (RUO).

Product Properties

Host Goat
Clonality Polyclonal
Conjugation Unconjugated
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.


Gene ID
Gene Symbol
Molecular Weight 54.4 kDa
Database Links
Alternative Names CHEK1Serine/threonine-protein kinase Chk1 CHEK1 antibodyCHK1
Function Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA, May also negatively regulate cell cycle progression during unperturbed cell cycles, This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome, Recognizes the substrate consensus sequence [R-X-X-S/T], Binds to and phosphorylates CDC25A, CDC25B and CDC25C, Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C, Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A, Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A, Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression, Also phosphorylates NEK6, Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination, Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation, Also promotes repair of DNA cross-links through phosphorylation of FANCE, Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A, This may enhance chromatin assembly both in the presence or absence of DNA damage, May also play a role in replication fork maintenance through regulation of PCNA, May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones, Phosphorylates histone H3,1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes, May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest
Cellular Localization Nucleus, Cytoplasm, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,
Tissue Specificity Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon,
Swiss-Prot Key


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