Anti-CHUK Antibody

Rs. 45,619.00
SKU stj113543

General Information

Product name Anti-CHUK Antibody
Short Description Rabbit polyclonal to CHUK
Description This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
Applications WB,IF
Dilution range WB 1:500 - 1:2000
IF 1:50 - 1:200
Protein Name Inhibitor of nuclear factor kappa-B kinase subunit alphaI-kappa-B kinase alphaIKK-AIKK-alphaIkBKAIkappaB kinase
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 516-745 of human CHUK (NP_001269.3).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Human,Mouse,Rat
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 84.6 kDa
Database Links
Alternative Names CHUKInhibitor of nuclear factor kappa-B kinase subunit alpha antibodyI-kappa-B kinase alpha antibodyIKK-A antibodyIKK-alpha antibodyIkBKA antibodyIkappaB kinaseCHUK antibodyIKKA antibodyTCF16
Function Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses, Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues, These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome, In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis, Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11), Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation, As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes, In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis, Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK, Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities, Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP, Additionally, phosphorylates the CREBBP-interacting protein NCOA3, Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor.
Cellular Localization Cytoplasm.
Tissue Specificity Widely expressed
Swiss-Prot Key

 

You recently viewed

Clear recently viewed