Anti-Cleaved-Caspase-8 (D384) antibody

Rs. 50,211.00
SKU stj90010

General Information

Product name Anti-Cleaved-Caspase-8 (D384) antibody
Short Description Rabbit polyclonal to Cleaved-Caspase-8 (D384).
Description Caspase-8 is a protein encoded by the CASP8 gene which is approximately 55,3 kDa. Caspase-8 is localised to the cytoplasm and is involved in the TNFR1 pathway, dimerization of procaspase-8 , activated TLR4 signalling, apoptosis signalling and toll-like receptor signalling pathways. This protein falls under the cysteine-aspartic acid protease family. It pays a role in the programmed cell death induced by Fas and various apoptotic stimuli. Caspase-8 isoform 1, 5 and 7 are expressed in a wide variety of tissues. Mutations in the CASP8 gene may result in a caspase-8 deficiency. stj90010 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of fragment activated Caspase-8 p18 protein resulting from cleavage adjacent to D384.
Applications WB,ELISA
Dilution range WB 1:500-1:2000ELISA 1:40000
Protein Name Anti-Cleaved-Caspase-8 (D384) antibody
Immunogen Synthesized peptide derived from human Caspase-8
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 47/55 kDa
Database Links
Alternative Names Cleaved-Caspase-8 (D384)
Caspase-8 antibody
CASP-8 antibody
Apoptotic cysteine protease antibody
Apoptotic protease Mch-5 antibody
CAP4 antibody
FADD-homologous ICE/ced-3-like protease antibody
FADD-like ICE antibody
FLICE antibody
ICE-like apoptotic protease 5 antibody
MORT1-associated ced-3 homolog antibody
MACH antibody
Caspase-8 subunit p18 antibody
Caspase-8 subunit p10 antibody
MCH5 antibody
Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Cellular Localization Cytoplasm.
Tissue Specificity Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
Swiss-Prot Key

 

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