Anti-DAPK3 antibody

Rs. 50,211.00
SKU stj92654

General Information

Product name Anti-DAPK3 antibody
Short Description Rabbit polyclonal to DAPK3.
Description DAPK3 is a protein encoded by the DAPK3 gene which is approximately 52,5 kDa. DAPK3 is localised to the nucleus and cytoplasm. It is involved in CDK-mediated phosphorylation and removal of Cdc6, dimerization of procaspase and pathways in cancer. It is a serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. It also plays a role in the regulation of smooth muscle contraction. DAPK3 is widely expressed in human tissues including the smooth muscle of the bladder. stj92654 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of DAPK3 protein.
Applications WB,IHC,ELISA,IF
Dilution range WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000
Protein Name Anti-DAPK3 antibody
Immunogen Synthesized peptide derived from human DAPK3 around the non-phosphorylation site of T265.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Human,Mouse,Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 52 kDa
Database Links
Alternative Names Death-associated protein kinase 3 antibody
DAP kinase 3 antibody
DAP-like kinase antibody
Dlk antibody
MYPT1 kinase antibody
Zipper-interacting protein kinase antibody
ZIP-kinase antibody
ZIPK antibody
Function Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.
Cellular Localization Nucleus Cytoplasm. Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm. cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. Isoform 1: Nucleus Cytoplasm Isoform 2: Nucleus Cytoplasm
Tissue Specificity Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle.
Swiss-Prot Key

 

You recently viewed

Clear recently viewed
Print Friendly and PDF