General Information
| Product name | Anti-EGFR antibody |
|---|---|
| Short Description | |
| Description | Rabbit polyclonal to EGFR. |
| Applications | WB,IHC,ELISA |
| Dilution range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000 |
| Protein Name | Anti-EGFR antibody |
| Immunogen | Synthesized peptide derived from human EGFR around the non-phosphorylation site of Y1092. |
| Storage Instruction | Store at -20°C, and avoid repeat freeze-thaw cycles. |
| Type of Usage | For Research Use Only (RUO). |
Product Properties
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Reactivity | Mouse,Rat |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Isotype | IgG |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Target
| Gene ID | |
|---|---|
| Gene Symbol | |
| Molecular Weight | 170 kDa |
| Database Links | |
| Alternative Names | Epidermal growth factor receptor antibody Proto-oncogene c-ErbB-1 antibody Receptor tyrosine-protein kinase erbB-1 antibody ERBB antibody ERBB1 antibody HER1 antibody |
| Function | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance . Isoform 2 may act as an antagonist of EGF action.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. |
| Cellular Localization | Cell membrane. Single-pass type I membrane protein. Endoplasmic reticulum membrane. Single-pass type I membrane protein. Golgi apparatus membrane. Single-pass type I membrane protein. Nucleus membrane. Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).. Isoform 2: Secreted. |
| Tissue Specificity | Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. |
| Swiss-Prot Key |