Anti-EIF2AK2 Antibody

Rs. 45,619.00
SKU stj27547

General Information

Product name Anti-EIF2AK2 Antibody
Short Description Rabbit polyclonal to EIF2AK2
Description The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.
Applications WB,IHC,IF
Dilution range WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Protein Name Anti-EIF2AK2 Antibody
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Eif2ak2 (NP_002750.1).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 62.1 kDa
Database Links
Alternative Names EIF2AK2Interferon-induced antibodydouble-stranded RNA-activated protein kinase EIF2AK2 antibodyPKR antibodyPRKR
Function IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation, Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1), Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis, Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11, In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation, Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs, Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6, Can act as both a positive and negative regulator of the insulin signaling pathway (ISP), Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2), Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes, Can trigger apoptosis via FADD-mediated activation of CASP8, Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin.
Cellular Localization Cytoplasm, Nucleus, Cytoplasm, perinuclear region.
Tissue Specificity Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues, colocalizes with gsk3b and tau in the alzheimer disease (ad) brain, elevated levels seen in breast and colon carcinomas,and which correlates with tumor progression and invasiveness or risk of progression.
Swiss-Prot Key

 

You recently viewed

Clear recently viewed