Anti-IL27 Antibody

Rs. 45,619.00
SKU stj27449

General Information

Product name Anti-IL27 Antibody
Short Description Rabbit polyclonal to IL27
Description The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).
Applications WB
Dilution range WB 1:500 - 1:2000
Protein Name Anti-IL27 Antibody
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-243 of human IL27 (NP_663634.2).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 27.5 kDa
Database Links
Alternative Names IL27Interleukin-27 subunit alpha antibodyIL-27 subunit alpha antibodyIL-27-A antibodyIL27-A antibodyInterleukin-30 antibodyp28IL27 antibodyIL27A antibodyIL30
Function Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity, IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells, Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17), It drives rapid clonal expansion of naive but not memory CD4 T-cells, It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction, IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation, It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways, It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment, It suppresses the expression of GATA3, the inhibitor TH1 cells development, In CD8 T-cells, it activates STATs as well as GZMB, IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production, Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells, While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1, IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3, Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells, Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9, In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II, IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.
Cellular Localization Secreted.
Tissue Specificity Expressed in monocytes and in placenta.
Swiss-Prot Key

 

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