Anti-NONO Antibody

Rs. 45,619.00
SKU stj27235

General Information

Product name Anti-NONO Antibody
Short Description Rabbit polyclonal to NONO
Description This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
Applications WB,IHC,IF
Dilution range WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Protein Name Anti-NONO Antibody
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NONO (NP_001138881.1).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 54.2 kDa
Database Links
Alternative Names NONONon-POU domain-containing octamer-binding protein antibodyNonO protein antibody54 kDa nuclear RNA- and DNA-binding protein antibody55 kDa nuclear protein antibodyDNA-binding p52/p100 complex antibody52 kDa subunit antibodyNMT55 antibodyp54(nrb antibody antibodyp54nrbNONO antibodyNRB54
Function DNA- and RNA binding protein, involved in several nuclear processes, Binds the conventional octamer sequence in double-stranded DNA, Also binds single-stranded DNA and RNA at a site independent of the duplex site, Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ, Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b, Together with PSPC1, required for the formation of nuclear paraspeckles, The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1, The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends, In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex, NONO is involved in transcriptional regulation, The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity, NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription, Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer, Important for the functional organization of GABAergic synapses, Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript, Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Cellular Localization Nucleus, Nucleus, nucleolus, Nucleus speckle.
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, also found in a number of breast tumor cell lines.
Swiss-Prot Key

 

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