Anti-Phospho-Chk2 (S516) antibody

Rs. 50,211.00
SKU stj90224

General Information

Product name Anti-Phospho-Chk2 (S516) antibody
Short Description
Description Rabbit polyclonal to Phospho-Chk2 (S516).
Applications WB,ELISA
Dilution range WB 1:500-1:2000ELISA 1:10000
Protein Name Anti-Phospho-Chk2 (S516) antibody
Immunogen Synthesized peptide derived from human Chk2 around the phosphorylation site of S516.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Monkey
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 61 kDa
Database Links
Alternative Names Phospho-Chk2 (S516)
Serine/threonine-protein kinase Chk2 antibody
CHK2 checkpoint homolog antibody
Cds1 homolog antibody
Hucds1 antibody
hCds1 antibody
Checkpoint kinase 2 antibody
CDS1 antibody
CHK2 antibody
RAD53 antibody
Function Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition .
Cellular Localization Isoform 2: Nucleus. Isoform 10 is present throughout the cell.. Isoform 4: Nucleus.. Isoform 7: Nucleus.. Isoform 9: Nucleus.. Isoform 12: Nucleus.. Nucleus, PML body. Nucleus, nucleoplasm. Recruited into PML bodies together with TP53.
Tissue Specificity High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
Swiss-Prot Key

 

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