Anti-Phospho-Histone H2A.X (S139) antibody

Rs. 50,211.00
SKU stj90288

General Information

Product name Anti-Phospho-Histone H2A.X (S139) antibody
Short Description Rabbit polyclonal to Phospho-Histone H2A.X (S139).
Description Histone H2A.X is a protein encoded by the H2AFX gene which is approximately 15,1 kDa. Histone H2A.X is localised to the nucleus. It is involved in DNA double-strand break repair and mitotic cell cycle. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units. Histone H2A.X is expressed in the lung, nervous system, intestine, skin and eye. Mutations in the H2AFX gene may result in Nijmegen breakage syndrome and ataxia-telangiectasia. stj90288 was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. This primary antibody specifically binds to endogenous Histone H2A.X protein which only binds about S139 when S139 is phosphorylated.
Applications WB,IHC,ELISA
Dilution range WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000
Protein Name Anti-Phospho-Histone H2A.X (S139) antibody
Immunogen Synthesized peptide derived from human Histone H2A.X around the phosphorylation site of S139.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Mouse,Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 15 kDa
Database Links
Alternative Names Phospho-Histone H2A.X (S139)
Histone H2AX antibody
H2a/x antibody
Histone H2A.X antibody
H2AX antibody
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Cellular Localization Nucleus Chromosome
Tissue Specificity
Swiss-Prot Key

 

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