Anti-Phospho-IKKα/β (S176/177) antibody

Rs. 50,211.00
SKU stj90301

General Information

Product name Anti-Phospho-IKKα/β (S176/177) antibody
Short Description Rabbit polyclonal to Phospho-IKKα/β (S176/177).
Description IKKα/β is a protein encoded by the CHUK gene which is approximately 84,6 kDa. IKKα/β is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the TNFR1 pathway, the 4-1BB pathway, RET signalling and toll-like receptor signalling pathways. This protein falls under the serine/threonine protein kinase family. It has an essential role in the NF-kappa-B signalling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. These modifications allow for degradation by the proteasome. IKKα/β is widely expressed. Mutations in the CHUK gene result in Cocoon syndrome and incontinentia pigmenti. stj90301 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous IKKα/β protein which only binds about S176/177 when S176/177 is phosphorylated.
Applications WB,IHC,ELISA
Dilution range WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000
Protein Name Anti-Phospho-IKKα/β (S176/177) antibody
Immunogen Synthesized peptide derived from human IKKα/β around the phosphorylation site of S176/177.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Mouse,Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 80 kDa
Database Links
Alternative Names Phospho-IKKa/β (S176/177)
Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
I-kappa-B kinase alpha antibody
IKK-A antibody
IKK-alpha antibody
IkBKA antibody
IkappaB kinase antibody
Conserved helix-loop-helix ubiquitous kinase antibody
I-kappa-B kinase 1 antibody
IKK1 antibody
Nuclear factor NF-kappa-B inhibitor kinase alpha antibody
NFKBIKA antibody
Transcription factor 16 antibody
TCF-16 antibody
IKKA antibody
TCF16 antibody
Function Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor .
Cellular Localization Cytoplasm Nucleus. Shuttles between the cytoplasm and the nucleus.
Tissue Specificity Widely expressed.
Swiss-Prot Key

 

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