Anti-Phospho-p53 (S15) antibody

Rs. 50,211.00
SKU stj90365

General Information

Product name Anti-Phospho-p53 (S15) antibody
Short Description Rabbit polyclonal to Phospho-p53 (S15).
Description p53 is a protein encoded by the TP53 gene which is approximately 43,6 kDa. p53 is localised to the cytoplasm, nucleus and endoplasmic reticulum. It is involved in RET signalling, CDK-mediated phosphorylation and removal of Cdc6, glioma and apoptotic pathways. It is a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. It responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest and changes in metabolism. p53 is expressed ubiquitously. Mutations in the TP53 gene may result in Li-Fraumeni syndrome and choroid plexus papilloma. stj90365 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous p53 protein which only binds about S15 when S15 is phosphorylated.
Applications WB,IHC,IP,IF,ELISA
Dilution range WB 1:500-1:2000IHC 1:100-1:300IP 1:200-500IF 1:200-1:1000ELISA 1:10000
Protein Name Anti-Phospho-p53 (S15) antibody
Immunogen Synthesized peptide derived from human p53 around the phosphorylation site of S15.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 48 kDa
Database Links
Alternative Names Phospho-p53 (S15)
Cellular tumor antigen p53 antibody
Antigen NY-CO-13 antibody
Phosphoprotein p53 antibody
Tumor suppressor p53 antibody
P53 antibody
Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 .
Cellular Localization Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2. Translocates to mitochondria upon oxidative stress. Translocates to mitochondria in response to mitomycin C treatment . Isoform 1: Nucleus. Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4.. Isoform 2: Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm.. Isoform 3: Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells.. Isoform 4: Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.. Isoform 7: Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm.. Isoform 8: Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli.. Isoform 9: Cytoplasm.
Tissue Specificity Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
Swiss-Prot Key

 

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