Anti-Phospho-p70 S6 kinase α (S371) antibody

Rs. 50,211.00
SKU stj90824

General Information

Product name Anti-Phospho-p70 S6 kinase α (S371) antibody
Short Description
Description Rabbit polyclonal to Phospho-p70 S6 kinase α (S371).
Applications WB,IHC,ELISA
Dilution range WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000
Protein Name Anti-Phospho-p70 S6 kinase α (S371) antibody
Immunogen Synthesized peptide derived from human p70 S6 kinase α around the phosphorylation site of S371.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Mouse,Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 60 kDa
Database Links
Alternative Names Phospho-p70 S6 kinase a (S371)
Ribosomal protein S6 kinase beta-1 antibody
S6K-beta-1 antibody
S6K1 antibody
70 kDa ribosomal protein S6 kinase 1 antibody
P70S6K1 antibody
p70-S6K 1 antibody
Ribosomal protein S6 kinase I antibody
Serine/threonine-protein kinase 14A antibody
p70 ribosomal S6 kinase alpha antibody
p70 S6 kinase alpha antibody
p70 S6K-alpha antibody
p70 S6KA antibody
STK14A antibody
Function Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.
Cellular Localization Cell junction, synapse, synaptosome Mitochondrion outer membrane. Mitochondrion. Colocalizes with URI1 at mitochondrion.. Isoform Alpha I: Nucleus. Cytoplasm.. Isoform Alpha II: Cytoplasm.
Tissue Specificity Widely expressed.
Swiss-Prot Key

 

You recently viewed

Clear recently viewed