Anti-Phospho-Rb (S612) antibody

Rs. 50,211.00
SKU stj91155

General Information

Product name Anti-Phospho-Rb (S612) antibody
Short Description
Description Rabbit polyclonal to Phospho-Rb (S612).
Applications IHC,ELISA
Dilution range IHC 1:100-1:300ELISA 1:5000
Protein Name Anti-Phospho-Rb (S612) antibody
Immunogen Synthesized peptide derived from human Rb around the phosphorylation site of S612.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 106 kDa
Database Links
Alternative Names Phospho-Rb (S612)
Retinoblastoma-associated protein antibody
p105-Rb antibody
pRb antibody
Rb antibody
pp110 antibody
Function Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex . In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Cellular Localization Nucleus
Tissue Specificity Expressed in the retina.
Swiss-Prot Key

 

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