Anti-Phospho-Sgo1 (S14) antibody

Rs. 50,211.00
SKU stj91267

General Information

Product name Anti-Phospho-Sgo1 (S14) antibody
Short Description
Description Rabbit polyclonal to Phospho-Sgo1 (S14).
Applications IHC,ELISA
Dilution range IHC 1:100-1:300ELISA 1:5000
Protein Name Anti-Phospho-Sgo1 (S14) antibody
Immunogen Synthesized peptide derived from human Sgo1 around the phosphorylation site of S14.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 64 kDa
Database Links
Alternative Names Phospho-Sgo1 (S14)
Shugoshin 1 antibody
Serologically defined breast cancer antigen NY-BR-85 antibody
Shugoshin-like 1 antibody
SGOL1 antibody
Function Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase.
Cellular Localization Nucleus Chromosome, centromere Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle pole Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase . Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A . Colocalizes with NEK2 at the kinetochore . Colocalizes with and SS18L1 at the kinetochore . Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore . Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis . Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent . Isoform 3 does not localize to kinetochores during any stages of the cell cycle .
Tissue Specificity Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested.
Swiss-Prot Key

 

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