Anti-Phospho-Smad3 (S213) antibody

Rs. 50,211.00
SKU stj90524

General Information

Product name Anti-Phospho-Smad3 (S213) antibody
Short Description
Description Rabbit polyclonal to Phospho-Smad3 (S213).
Applications WB,IHC,ELISA
Dilution range WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000
Protein Name Anti-Phospho-Smad3 (S213) antibody
Immunogen Synthesized peptide derived from human Smad3 around the phosphorylation site of S213.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Human,Mouse,Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 48 kDa
Database Links
Alternative Names Phospho-Smad3 (S213)
Mothers against decapentaplegic homolog 3 antibody
MAD homolog 3 antibody
Mad3 antibody
Mothers against DPP homolog 3 antibody
hMAD-3 antibody
JV15-2 antibody
SMAD family member 3 antibody
SMAD 3 antibody
Smad3 antibody
hSMAD3 antibody
MADH3 antibody
Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Localization Cytoplasm Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 . Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 . Co-localizes with LEMD3 at the nucleus inner membrane . MAPK-mediated phosphorylation appears to have no effect on nuclear import . PDPK1 prevents its nuclear translocation in response to TGF-beta .
Tissue Specificity
Swiss-Prot Key

 

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