Anti-Phospho-Smad3 (S425) antibody

Rs. 50,211.00
SKU stj90745

General Information

Product name Anti-Phospho-Smad3 (S425) antibody
Short Description Rabbit polyclonal to Phospho-Smad3 (S425).
Description Smad3 is a protein encoded by the SMAD3 gene which is approximately 48 kDa. Smad3 is localised to the cytoplasm and nucleus. It is involved in SMAD2/3 MH2 domain mutants in cancer, the cell cycle and toll-like receptor signalling pathway. It an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. It binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Smad3 is expressed in the nervous system, pancreas, lung, kidney and intestine. Mutations in the SMAD3 gene may result in colorectal cancer and Loeys-Dietz syndrome. stj90745 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Smad3 protein which only binds about S425 when S425 is phosphorylated.
Applications WB,IHC,ELISA
Dilution range WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000
Protein Name Anti-Phospho-Smad3 (S425) antibody
Immunogen Synthesized peptide derived from human Smad3 around the phosphorylation site of S425.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Mouse,Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 50 kDa
Database Links
Alternative Names Phospho-Smad3 (S425)
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Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Localization Cytoplasm Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 . Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 . Co-localizes with LEMD3 at the nucleus inner membrane . MAPK-mediated phosphorylation appears to have no effect on nuclear import . PDPK1 prevents its nuclear translocation in response to TGF-beta .
Tissue Specificity
Swiss-Prot Key

 

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