Anti-Phospho-Tie-2 (Y992) antibody

Rs. 50,211.00
SKU stj90597

General Information

Product name Anti-Phospho-Tie-2 (Y992) antibody
Short Description
Description Rabbit polyclonal to Phospho-Tie-2 (Y992).
Applications WB,ELISA
Dilution range WB 1:500-1:2000ELISA 1:10000
Protein Name Anti-Phospho-Tie-2 (Y992) antibody
Immunogen Synthesized peptide derived from human Tie-2 around the phosphorylation site of Y992.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity Mouse
Conjugation Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Target

Gene ID
Gene Symbol
Molecular Weight 120 kDa
Database Links
Alternative Names Phospho-Tie-2 (Y992)
Angiopoietin-1 receptor antibody
Endothelial tyrosine kinase antibody
Tunica interna endothelial cell kinase antibody
Tyrosine kinase with Ig and EGF homology domains-2 antibody
Tyrosine-protein kinase receptor TEK antibody
Tyrosine-protein kinase receptor TIE-2 antibody
hTIE2 antibody
p140 TEK antibody
CD antigen CD202b antibody
TIE2 antibody
VMCM antibody
VMCM1 antibody
Function Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1.
Cellular Localization Cell membrane Cell junction, focal adhesion Cytoplasm, cytoskeleton. Secreted. Recruited to cell-cell contacts in quiescent endothelial cells . Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted .
Tissue Specificity Detected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma (at protein level). Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
Swiss-Prot Key

 

You recently viewed

Clear recently viewed