Anti-PML Antibody

Rs. 45,619.00
SKU stj25034

General Information

Product name Anti-PML Antibody
Short Description Rabbit polyclonal to PML
Description The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
Applications WB,IHC,IF
Dilution range WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Protein Name Anti-PML Antibody
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150253.2).
Storage Instruction Store at -20℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 97.6 kDa
Database Links
Alternative Names PMLProtein PML antibodyPromyelocytic leukemia protein antibodyRING finger protein 71 antibodyTripartite motif-containing protein 19PML antibodyMYL antibodyPP8675 antibodyRNF71 antibodyTRIM19
Function Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms, Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions, Isoform PML-4 has a multifaceted role in the regulation of apoptosis and growth suppression: activates RB1 and inhibits AKT1 via interactions with PP1 and PP2A phosphatases respectively, negatively affects the PI3K pathway by inhibiting MTOR and activating PTEN, and positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation), Isoform PML-4 also: acts as a transcriptional repressor of TBX2 during cellular senescence and the repression is dependent on a functional RBL2/E2F4 repressor complex, regulates double-strand break repair in gamma-irradiation-induced DNA damage responses via its interaction with WRN, acts as a negative regulator of telomerase by interacting with TERT, and regulates PER2 nuclear localization and circadian function, Isoform PML-6 inhibits specifically the activity of the tetrameric form of PKM, The nuclear isoforms (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5) in concert with SATB1 are involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus, Isoform PML-2 is required for efficient IFN-gamma induced MHC II gene transcription via regulation of CIITA, Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway, PML also regulates transcription activity of ELF4 and can act as an important mediator for TNF-alpha- and IFN-alpha-mediated inhibition of endothelial cell network formation and migration
Cellular Localization Nucleus, Nucleus, nucleoplasm, Cytoplasm, Nucleus, PML body, Early endosome membrane
Tissue Specificity
Swiss-Prot Key

 

You recently viewed

Clear recently viewed
Print Friendly and PDF