Anti-PUF60 Antibody

Rs. 61,689.00
SKU stj25238

General Information

Product name Anti-PUF60 Antibody
Short Description Rabbit polyclonal to PUF60
Description This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Applications WB
Dilution range WB 1:500 - 1:2000
Protein Name Anti-PUF60 Antibody
Immunogen A synthetic peptide of human PUF60
Storage Instruction Store at 4℃. Avoid freeze / thaw cycles.
Type of Usage For Research Use Only (RUO).

Product Properties

Host Rabbit
Clonality Polyclonal
Reactivity
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation Buffer: PBS with 0.02% sodium azide, pH7.3.

Target

Gene ID
Gene Symbol
Molecular Weight 59.9 kDa
Database Links
Alternative Names PUF60Poly(U antibody-binding-splicing factor PUF60 antibody60 kDa poly(U antibody-binding-splicing factor antibodyFUSE-binding protein-interacting repressor antibodyFBP-interacting repressor antibodyRo-binding protein 1 antibodyRoBP1 antibodySiah-binding protein 1 antibodySiah-BP1PUF60 antibodyFIR antibodyROBPI antibodySIAHBP1
Function DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation, In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor, Acts as a transcriptional repressor through the core-TFIIH basal transcription factor, Represses FUBP1-induced transcriptional activation but not basal transcription, Decreases ERCC3 helicase activity, Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells, Is also involved in pre-mRNA splicing, Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2, Involved in apoptosis induction when overexpressed in HeLa cells, Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer, Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells, Modulates alternative splicing of several mRNAs, Binds to relaxed DNA of active promoter regions, Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA
Cellular Localization Nucleus.
Tissue Specificity Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level), isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium, expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Swiss-Prot Key

 

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