Canvax SNP Taq DNA Polymerase

Rs. 18,943.10
SKU

Highly efficient Hot-Start Polymerase for SNP detection by allele-specific PCR and micro sequencing

Make: Canvax Bio

Cat. No: SC001

Product Overview:

SNP Taq DNA Polymerase is an highly efficient, High Fidelity and specific Hot-Start Polymerase with special N-terminal deletion and proprietary amino acids substitutions introduced into the active center of the enzyme.

Due this special modification, the enzyme increases its sensitivity to mismatches at 3’-end of the primer. For this reason, unspecific amplicons are formatted due to the non-perfect annealing of the primers.

SNP Taq DNA Polymerase lack of 5′ -exonuclease activity.

Advantages & Features:

  • Efficient: 10 to 15-fold lower mutation rate than normal Taq DNA Polymerase.
  • High fidelity allele-specific amplification of DNA fragments.
  • High Specificity: lowest background AS-PEX and AS-PCR.
  • Hot-Start activity for less primer dimers.
  • Only 5′-3′ polymerase activity, lack of 5’-exonuclease activity.
  • Risk-free: product covered by our Quality 100% Guarantee

Specifications: 

  • Unit definition: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72 °C.

Includes:

Includes for 500 U:

SNP Taq DNA Polymerase

Reaction Buffer (5x) without MgCL2

– 100 mM MgCl2

Applications:

  • High specific or Multiplex PCR.
  • Real-Time PCR with intercalation dyes.
  • High Fidelity dNTPs and ddNTPs.
  • Mini Sequencing procedures.
  • Allele-specific primer extension (AS-PEX).
  • SNP genotyping by allele-specific PCR (AS-PCR).
  • Single Nucleotide Polymorphism (SNP).

Data Sheet

Brochure

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