ELISA Kit for Fibroblast Growth Factor 6 (FGF6) - Human

by USCNK
Rs. 40,950.00
SKU undefined
Description: 96 well plate
Catalog No: RS01E0675

Organism species : Homo sapiens (Human)

Sample type : Serum, plasma and other biological fluids.

Format : 96-well strip plate

Assay length : 4.5 hours

Detection range : 

  • 7.81-500pg/mL The standard curve concentrations used for the ELISA’s were 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL, 7.81pg/mL

Sensitivity : 

  • The minimum detectable dose of this kit is typically less than   3.3pg/mL.
Specificity :
  • This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 6 (FGF6).
    No significant cross-reactivity or interference between Fibroblast Growth Factor 6 (FGF6) and analogues was observed.
Precision :
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested 20 times on one plate, respectively. 
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested on 3 different plates, 8 replicates in each plate. 
  • CV(%) = SD/meanX100 
  • Intra-Assay: CV<10% 
  • Inter-Assay: CV<12%
                  Stability :
                  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested 20 times on one plate, respectively. 
                  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested on 3 different plates, 8 replicates in each plate. 
                  • CV(%) = SD/meanX100 
                  • Intra-Assay: CV<10% 
                  • Inter-Assay: CV<12%
                    Reagents and materials provided :
                    Reagents Quantity Reagents Quantity
                    Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
                    Standard 2 Standard Diluent 1×20mL
                    Detection Reagent A 1×120µL Assay Diluent A 1×12mL
                    Detection Reagent B 1×120µL Assay Diluent B 1×12mL
                    TMB Substrate 1×9mL Stop Solution 1×6mL
                    Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

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