ELISA Kit for Matrix Metalloproteinase 13 (MMP13) - Mouse

by USCNK
Rs. 42,120.00
SKU undefined
Description: 96 well plate
Catalog No: RS01E0999

Organism species : Mus musculus (Mouse)

Sample type : Serum, plasma and other biological fluids.

Format : 96-well strip plate

Assay length : 4.5 hours

Detection range : 

  • 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL

Sensitivity : 

  • The minimum detectable dose of this kit is typically less than 6.5pg/mL.
Specificity :
  • This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 13 (MMP13).
    No significant cross-reactivity or interference between Matrix Metalloproteinase 13 (MMP13) and analogues was observed.
Precision :
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested 20 times on one plate, respectively. 
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested on 3 different plates, 8 replicates in each plate. 
  • CV(%) = SD/meanX100 
  • Intra-Assay: CV<10% 
  • Inter-Assay: CV<12%
                                Stability :
                                • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested 20 times on one plate, respectively. 
                                • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested on 3 different plates, 8 replicates in each plate. 
                                • CV(%) = SD/meanX100 
                                • Intra-Assay: CV<10% 
                                • Inter-Assay: CV<12%
                                  Reagents and materials provided :
                                  Reagents Quantity Reagents Quantity
                                  Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
                                  Standard 2 Standard Diluent 1×20mL
                                  Detection Reagent A 1×120µL Assay Diluent A 1×12mL
                                  Detection Reagent B 1×120µL Assay Diluent B 1×12mL
                                  TMB Substrate 1×9mL Stop Solution 1×6mL
                                  Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

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