Food Vitamin B12 ELISA Kit

Rs. 33,000.00

Make: Cortez Diagnostics

Vitamin B12 quantitative ELISA test is based on the principle of the enzyme-linked immunosorbent assay for Quantitative determination of Vitamin B12 in Food.

Materials Provided with Food Vitamin B12 Elisa Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated with anti-vitamin B12
2. Vitamin B12 Standards (0; 0.4; 1; 4; 10; 40 ng/mL): 6 vials with 0.5 mL each
3. Conjugate (Vitamin B12-Peroxidase): 6 mL
4. Substrate Solution (TMB): 15 mL
5. Stop Solution (0.5 M H2SO4): 15 mL
6. Sample diluent (PBS) 2x 
7. Washing Solution (PBS + Tween 20): 60 mL as 10x 
8. Two plastic foils to cover the strips during the incubation 
9. Plastic bag to store unused microtiter strips
10. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette 
3. EIA kit Microplate washer 
4. EIA kit Microplate Reader with 450nm wavelength

Vitamin B12 Elisa Kit Background Information:
Vitamin B12 as a trace element belongs to the biologically important chelate formers. The basic unit consists of a corrin ring with cobalt as a central atom. Cobalt is sixfold coordinated by four nitrogen atoms, one cyanide, and a dimethyl benzimidazole group. Vitamin B12 forms a stable complex, which is absorbed in the lower part of the small intestine, with the so-called intrinsic factor present in the gastric juice. A lack of vitamin B12 can lead among other things to pernicious anemia. This disease is not generated by an insufficient supply of vitamin B12, but by the absence of intrinsic factor. Pernicious anemia can be treated by a high dosage of vitamin B12. The existing detection procedures are mainly microbiological methods, but also HPLC and thin-layer chromatography, all of which are associated with a high amount of time and instrumentation. With the present test kit, it is possible, to determine vitamin B12 quantitatively in vitaminated food in a significantly faster way (2.5 to 4 hours inclusive sample pretreatment) compared with a conventional microbiological assay (24 to 48 hours).

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