High Sensitive ELISA Kit for Immunoglobulin G (IgG) - Human

Rs. 22,360.00
SKU undefined
Description: 96 well plate
Catalog No: RS01E0526

Organism species : Homo sapiens (Human)

Sample type : Serum, plasma and other biological fluids.

Format : 96-well strip plate

Assay length : 4.5 hours

Detection range : 

  • 1.56-100pg/mL The standard curve concentrations used for the ELISA’s were 100pg/mL, 50pg/mL, 25pg/mL, 12.5pg/mL, 6.25pg/mL, 3.13pg/mL, 1.56pg/mL

Sensitivity : 

  • The minimum detectable dose of this kit is typically less than  0.55pg/mL.
Specificity :
  • This assay has high sensitivity and excellent specificity for detection of High Sensitive Tumor Necrosis Factor Alpha (TNFa).
    No significant cross-reactivity or interference between High Sensitive Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
Precision :
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Tumor Necrosis Factor Alpha (TNFa) were tested 20 times on one plate, respectively. 
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Tumor Necrosis Factor Alpha (TNFa) were tested on 3 different plates, 8 replicates in each plate. 
  • CV(%) = SD/meanX100 
  • Intra-Assay: CV<10% 
  • Inter-Assay: CV<12%
        Stability :
        • The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
        • To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
        Reagents and materials provided :
        Reagents Quantity Reagents Quantity
        Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
        Standard 2 Standard Diluent 1×20mL
        Detection Reagent A 1×120µL Assay Diluent A 1×12mL
        Detection Reagent B 1×120µL Assay Diluent B 1×12mL
        TMB Substrate 1×9mL Stop Solution 1×6mL
        Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

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