Pg(Progesterone) ELISA Kit
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Goat Anti-Rabbit IgG, make solid-phase secondary antibody. And then add samples, horseradish peroxidase-labeled Progesterone and anti- Progesterone antibody, so as to form a coated secondary antibody - anti- Progesterone antibody - HRP-labeled Progesterone complex. The amount of bound labeled Progesterone is inversely proportional to that of Progesterone in the samples. The TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Progesterone in the samples is then determined by comparing the OD of the samples to the standard curve.
|Format / Size||96T|
|Reactivity / Target Species||Universal|
|Sample type||Serum, Plasma, Cell supernatant|