Monoclonal Antibody to Immunoglobulin G2 (IgG2) - Human

by USCNK
Rs. 34,320.00
SKU

Catalog No :RS01Ab0248

Organism- Human

Clonality :Monoclonal

Host :Mouse

Concentration :500ug/ml

Application :WB, ICC, IHC-P, IHC-F, ELISA

Immunoglobulin Type :IgG

Purification :Affinity Chromatography

Conjugate :No Conjugate

IMMUNOGEN INFORMATION
Immunogen:
  • Native Protein IgG2.
  • There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
  • Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, IgG2-B, and IgG2-A/B, which differ by the disulfide connectivity at the hinge region. IgG2, the second largest part of IgG isotypes in humans, comprises 20-25% of the main subclass and is the prevalent immune response against carbohydrate-/polysaccharid antigens.
ANTIBODY SPECIFICITY
  • The antibody is a mouse monoclonal antibody raised against IgG2.
  • It has been selected for its ability to recognize IgG2 in immunohistochemical staining and western blotting.
APPLICATIONS
  • Western blotting: 1:100-400
  • Immunocytochemistry in formalin fixed cells: 1:100-500
  • Immunohistochemistry in formalin fixed frozen section: 1:100-500
  • Immunohistochemistry in paraffin section: 1:50-200
  • Enzyme-linked Immunosorbent Assay: 1:100-200
  • Optimal working dilutions must be determined by end user.
CONTENTS: Form & Buffer:
  • Supplied as solution form in PBS,
  • pH7.4,
  • containing 0.02% NaN3,
  • 50% glycerol.
STORAGE
  • Store at 4oC for frequent use.
  • Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity.
  • Avoid repeated freeze-thaw cycles.

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