Monoclonal Antibody to Immunoglobulin G3 (IgG3) - Human
Catalog No :RS01Ab0182
Application :WB, ICC, IHC-P, IHC-F, ELISA
Immunoglobulin Type: IgG
Purification: Affinity Chromatography
Conjugate: No Conjugate
Immunogen:Native Protein IgG3 from Human Serum
There are four immunoglobulin G subclasses, each numbered for their relative abundance in the body. These molecules are an important part of the immune system. All immunoglobulin G molecules are made up of four chains of amino acids, connected at a central hinge in such a way that three ends extend from the center. The hinge within the immunoglobulin G3 antibody is by far the longest in all the immunoglobulin G subclasses. The length of the hinge allows immunoglobulin G3 to quickly and effectively bind to and eliminate antigens. On the other hand, the length of the hinge also makes this subclass of immunoglobulin G the most susceptible to damage from other molecules.
The antibody is a mouse monoclonal antibody raised against IgG3. It has been selected for its ability to recognize IgG3 in immunohistochemical staining and western blotting.
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.