Cortisol ELISA Kit

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Rs. 49,500.00
Rs. 39,991.00
SKU E-EL-0030

Test Principle:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cortisol. During the reaction, Cortisol in the sample or standard competes with a fixed amount of Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type Competitive
Format / Size 96T
Assay time  
Reactivity / Target Species  Universal
Detection method Colormetric
Detection range 12.5~800ng/mL
Sensitivity 7.5ng/mL
Sample volume  
Sample type Serum, Plasma, Cell supernatant
Repeatability  
Target Synonym
Target Name Cortisol

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