Rat 20S-PSM(20S-Proteasome) ELISA Kit

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Rs. 58,000.00
Rs. 39,991.00
SKU E-EL-R1003

Test Principle:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat 20S-PSM. During the reaction, Rat 20S-PSM in the sample or standard competes with a fixed amount of Rat 20S-PSM on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat 20S-PSM. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type Competitive
Format / Size 96T
Assay time  
Reactivity / Target Species  Rat
Detection method Colormetric
Detection range 1.56~100ng/mL
Sensitivity 0.94ng/mL
Sample volume  
Sample type Serum, Plasma, Cell supernatant
Target Synonym CP, 20SPSM, 20S Core Particle
Target Name 20S-PSM

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