Rat BSP(Bone Sialoprotein) ELISA Kit

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Rs. 58,000.00
Rs. 39,991.00
SKU E-EL-R0244

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BSP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BSP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BSP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BSP. You can calculate the concentration of Rat BSP in the samples by comparing the OD of the samples to the standard curve.

Assay type Sandwich
Format / Size 96T
Assay time  
Reactivity / Target Species  Rat
Detection method Colormetric
Detection range 0.78~50ng/mL
Sensitivity 0.47ng/mL
Sample volume  
Sample type Serum, Plasma, Cell supernatant
Repeatability  
Target Synonym IBSP, BNSP, BSP, BSP-II, SP-II, Integrin-Binding Bone Sialoprotein
Target Name BSP

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