Rat NEFH(Neurofilament,HeavyPolypeptide) ELISAKit

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Rs. 58,000.00
Rs. 39,991.00
SKU E-EL-R2577

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NEFH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NEFH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NEFH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NEFH. You can calculate the concentration of Rat NEFH in the samples by comparing the OD of the samples to the standard curve.

Assay type Sandwich
Format / Size 96T
Assay time  
Reactivity / Target Species  Rat
Detection method Colormetric
Detection range 31.25~2000pg/mL
Sensitivity 18.75pg/mL
Sample volume  
Sample type Serum, Plasma, Cell supernatant
Repeatability  
Target Synonym
Target Name NEFH

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