Polyclonal Antibody to Activated Leukocyte Cell Adhesion Molecule (ALCAM) - Human

by USCNK
Rs. 14,378.00
SKU

Catalog No :RS01Ab0400

Organism- Human

Clonality :Polyclonal

Host :Rabbit

Concentration :200ug/ml

Application :WB, ICC, IHC-P, IHC-F, ELISA

Immunoglobulin Type :IgG

Purification :Affinity Chromatography

Conjugate :No Conjugate

IMMUNOGEN INFORMATION
  • Immunogen: Recombinant ALCAM (Trp28~Lys527) expressed in E.coli.
ANTIBODY SPECIFICITY
  • The antibody is a rabbit polyclonal antibody raised against ALCAM.
  • It has been selected for its ability to recognize ALCAM in immunohistochemical staining and western blotting.

APPLICATIONS

  • Western blotting: 1:100-400
  • Immunocytochemistry in formalin fixed cells: 1:100-500
  • Immunohistochemistry in formalin fixed frozen section: 1:100-500
  • Immunohistochemistry in paraffin section: 1:50-200
  • Enzyme-linked Immunosorbent Assay: 1:100-200
  • Optimal working dilutions must be determined by end user.

CONTENTS: Form & Buffer:

  • Supplied as solution form in PBS,
  • pH7.4,
  • containing 0.02% NaN3,
  • 50% glycerol

QUALITY CONTROL

  • Content:The quality control contains recombinant ALCAM (Trp28~Lys527) disposed in loading buffer
  • Usage:10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                    5uL per well when used in enhanced chemilumescent (ECL).
  • Note:The quality control is specifically manufactured as the positive control. Not used for other purposes.
  • Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

STORAGE

  • Store at 4oC for frequent use.
  • Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity.
  • Avoid repeated freeze-thaw cycles.

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