Polyclonal Antibody to Angiopoietin 1 (ANGPT1) - Rat

by USCNK
Rs. 15,561.00
SKU

Catalog No :RS01Ab0421

Organism- Rat

Clonality :Polyclonal

Host :Rabbit

Concentration :200ug/ml

Application :WB, ICC, IHC-P, IHC-F, ELISA

Immunoglobulin Type :IgG

Purification :Affinity Chromatography

Conjugate :No Conjugate

IMMUNOGEN INFORMATION
  • Immunogen: Recombinant ANGPT1 (Asn304~Ala450) expressed in E.coli.
ANTIBODY SPECIFICITY
  • The antibody is a rabbit polyclonal antibody raised against ANGPT1.
  • It has been selected for its ability to recognize ANGPT1 in immunohistochemical staining and western blotting.

APPLICATIONS

  • Western blotting: 1:100-400
  • Immunocytochemistry in formalin fixed cells: 1:100-500
  • Immunohistochemistry in formalin fixed frozen section: 1:100-500
  • Immunohistochemistry in paraffin section: 1:50-200
  • Enzyme-linked Immunosorbent Assay: 1:100-200
  • Optimal working dilutions must be determined by end user.

CONTENTS: Form & Buffer:

  • Supplied as solution form in PBS,
  • pH7.4,
  • containing 0.02% NaN3,
  • 50% glycerol

QUALITY CONTROL

Content:

  • The quality control contains recombinant ANGPT1 (Asn304~Ala450) disposed in loading buffer.

Usage:

  • 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  •  5uL per well when used in enhanced chemilumescent (ECL).

Note:

  • The quality control is specifically manufactured as the positive control.
  • Not used for other purposes.

Loading Buffer:

  • 100mM Tris(pH8.8),
  • 2% SDS,
  • 200mM NaCl,
  • 50% glycerol,
  • BPB 0.01%,
  • NaN3 0.02%.

STORAGE

  • Store at 4oC for frequent use.
  • Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity.
  • Avoid repeated freeze-thaw cycles.

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