Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE) - Mouse

Rs. 13,104.00

Catalog No :RS01Ab0407

Organism- Mouse

Clonality :Polyclonal

Host :Rabbit

Concentration :200ug/ml

Application :WB, ICC, IHC-P, IHC-F, ELISA

Immunoglobulin Type :IgG

Purification :Affinity Chromatography

Conjugate :No Conjugate

  • Immunogen: Recombinant ACE (Tyr504~Asp597) expressed in E.coli.
  • The antibody is a rabbit polyclonal antibody raised against ACE.
  • It has been selected for its ability to recognize ACE in immunohistochemical staining and western blotting.


  • Western blotting: 1:100-400
  • Immunocytochemistry in formalin fixed cells: 1:100-500
  • Immunohistochemistry in formalin fixed frozen section: 1:100-500
  • Immunohistochemistry in paraffin section: 1:50-200
  • Enzyme-linked Immunosorbent Assay: 1:100-200
  • Optimal working dilutions must be determined by end user.

CONTENTS: Form & Buffer:

  • Supplied as solution form in PBS,
  • pH7.4,
  • containing 0.02% NaN3,
  • 50% glycerol


  • Content:The quality control contains recombinant ACE (Tyr504~Asp597) disposed in loading buffer.
  • Usage:10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                 5uL per well when used in enhanced chemilumescent (ECL).
  • Note:The quality control is specifically manufactured as the positive control. Not used for other purposes.
  • Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.


  • Store at 4oC for frequent use.
  • Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity.
  • Avoid repeated freeze-thaw cycles.

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