Polyclonal Antibody to Factor Related Apoptosis Ligand (FASL) - Rat

by USCNK
Rs. 15,561.00
SKU

Catalog No :RS01Ab0511

Organism- Rat

Clonality :Polyclonal

Host :Rabbit

Application :WB, ICC, IHC-P, IHC-F, ELISA

Concentration : 200ug/ml

UOM : 100ug

Conjugate : No Conjugate

Immunogen Information : 

  • Immunogen : Recombinant FASL (Gln100~Leu278) expressed in E.coli.

    Antibody Specificity :

    The antibody is a rabbit polyclonal antibody raised against FASL. It has been selected for its ability to recognize FASL in immunohistochemical staining and western blotting.

    Applications :

    • Western blotting: 1:100-400
    • Immunocytochemistry in formalin fixed cells: 1:100-500
    • Immunohistochemistry in formalin fixed frozen section: 1:100-500
    • Immunohistochemistry in paraffin section: 1:50-200
    • Enzyme-linked Immunosorbent Assay: 1:100-200
    • Optimal working dilutions must be determined by end user.

    Contents :

    • Form & Buffer: Supplied as solution form in PBS, pH7.4,
    • containing 0.02% NaN3, 50% glycerol.

    Quality Control :

    • Content: The quality control contains recombinant FASL (Gln100~Leu278) disposed in loading buffer.
    • Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
    •                 5uL per well when used in enhanced chemilumescent (ECL).
    • Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
    • Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

    Storage :

    • Store at 4oC for frequent use.
    • Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity.
    • Avoid repeated freeze-thaw cycles.

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