Polyclonal Antibody to Fibronectin (FN) - Cattle

by USCNK
Rs. 10,283.00
SKU

Catalog No :RS01Ab0527

Organism- Cattle

Clonality :Polyclonal

Host :Rabbit

Application :WB, ICC, IHC-P, IHC-F, ELISA

Concentration : 200ug/ml

UOM : 100ug

Conjugate : No Conjugate

Immunogen Information : 

  • Immunogen : Native Protein Fibronectin
    Fibronectin is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Fibronectin exists as a protein dimer, consisting of two nearly identical monomers linked by a pair of disulfide bonds. The fibronectin protein is produced from a single gene, but alternative splicing of its pre-mRNA leads to the creation of several isoforms. There are two types of fibronectin present in vertebrates, soluble plasma fibronectin and insoluble cellular fibronectin. Fibronectin plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development.

Antibody Specificity :

The antibody is a rabbit polyclonal antibody raised against FN. It has been selected for its ability to recognize FN in immunohistochemical staining and western blotting.

Applications :

  • Western blotting: 1:100-400
  • Immunocytochemistry in formalin fixed cells: 1:100-500
  • Immunohistochemistry in formalin fixed frozen section: 1:100-500
  • Immunohistochemistry in paraffin section: 1:50-200
  • Enzyme-linked Immunosorbent Assay: 1:100-200
  • Optimal working dilutions must be determined by end user.

Contents :

  • Form & Buffer: Supplied as solution form in PBS, pH7.4,
  • containing 0.02% NaN3, 50% glycerol.

    Storage :

    • Store at 4oC for frequent use.
    • Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity.
    • Avoid repeated freeze-thaw cycles.

     

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