Polyclonal Antibody to Growth Hormone (GH) - Mouse

by USCNK
Rs. 14,742.00
SKU

Catalog No :RS01Ab0549

Organism- Mouse

Host :Rabbit

Application :WB, ICC, IHC-P, IHC-F, ELISA

Concentration : 200ug/ml

UOM : 100ug

Conjugate : No Conjugate

Immunogen Information : 

  • Immunogen :  Recombinant growth hormone (Pro28~Phe216) expressed in E.coli.

Antibody Specificity :

The antibody is a rabbit polyclonal antibody raised against GH. It has been selected for its ability to recognize GH in immunohistochemical staining and western blotting.

Applications :

  • Western blotting: 1:100-400
  • Immunocytochemistry in formalin fixed cells: 1:100-500
  • Immunohistochemistry in formalin fixed frozen section: 1:100-500
  • Immunohistochemistry in paraffin section: 1:50-200
  • Enzyme-linked Immunosorbent Assay: 1:100-200
  • Optimal working dilutions must be determined by end user.

Contents :

  • Form & Buffer: Supplied as solution form in PBS, pH7.4,
  • containing 0.02% NaN3, 50% glycerol.

Quality Control :

  • Content: The quality control contains recombinant growth hormone (Pro28~Phe216) disposed in loading buffer.
  • Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  •                 5uL per well when used in enhanced chemilumescent (ECL).
  • Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
  • Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

Storage :

  • Store at 4oC for frequent use.
  • Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity.
  • Avoid repeated freeze-thaw cycles.

 

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