Polyclonal Antibody to Macrophage Inflammatory Protein 1 Beta (MIP1b) - Mouse

Catalog No :RS01Ab0727

Organism- Mouse

Clonality :Polyclonal

Host :Rabbit

Application :WB, ICC, IHC-P, IHC-F, ELISA

Concentration : 200ug/ml

UOM : 100ug

Conjugate : No Conjugate

Immunogen Information : 

• Immunogen :  Recombinant MIP1b (Ala24~Asn92) expressed in E.coli.

Antibody Specificity :

The antibody is a rabbit polyclonal antibody raised against MIP1b. It has been selected for its ability to recognize MIP1b in immunohistochemical staining and western blotting.

Applications :

• Western blotting: 1:100-400
• Immunocytochemistry in formalin fixed cells: 1:100-500
• Immunohistochemistry in formalin fixed frozen section: 1:100-500
• Immunohistochemistry in paraffin section: 1:50-200
• Enzyme-linked Immunosorbent Assay: 1:100-200
• Optimal working dilutions must be determined by end user.

Contents :

• Form & Buffer: Supplied as solution form in PBS, pH7.4,
• containing 0.02% NaN3, 50% glycerol.

Quality Control :

• Content: The quality control contains recombinant MIP1b (Ala24~Asn92) disposed in loading buffer.
• Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
•                 5uL per well when used in enhanced chemilumescent (ECL).
• Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
• Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

Storage :

• Store at 4^oC for frequent use.
• Stored at -20^oC to -80^oC in a manual defrost freezer for one year without detectable loss of activity.
• Avoid repeated freeze-thaw cycles.