Recombinant Activated Leukocyte Cell Adhesion Molecule (ALCAM) - Mouse

by USCNK
Rs. 26,000.00
SKU

Catalog no : RS01P0277

Application : SDS-PAGE; WB; ELISA; IP.

Organism : Mouse

Purity : > 95%

Host : E.coli

Expression System : Prokaryotic expression

Mol. Mass : 59.9kDa

Chemical Formula : n/a

Rsidues : Trp28~Lys527 (Accession # Q61490) with two N-terminal Tags, His-tag and T7-tag

Assembled by : Uscn Life Science Inc.Wuhan

Formulation : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl

Usage : Reconstitute in sterile PBS, pH7.2-pH7.4.

Sequences : The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WYT VNSAYGDTIV MPCRLDVPQN LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL SLSENYTLSI ANAKISDEKR FVCMLVTEDN VFEAPTLVKV FKQPSKPEIV NKAPFLETDQ LKKLGDCISR DSYPDGNITW YRNGKVLQPV EGEVAILFKK EIDPGTQLYT VTSSLEYKTT RSDIQMPFTC SVTYYGPSGQ KTIYSEQEIF DIYYPTEQVT IQVLPPKNAI KEGDNITLQC LGNGNPPPEE FMFYLPGQPE GIRSSNTYTL TDVRRNATGD YKCSLIDKRN MAASTTITVH YLDLSLNPSG EVTKQIGDTL PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIHWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV SAISIPEHDE ADDISDENRE KVNDQAK

Storage and Stability :

  • Storage : Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
  • Stability Test : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the Marker (complimentary) :

  • Effective Size Range : 10kDa to 70kDa.
  • Protein bands : 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
  • Double intensity bands : The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
  • Ready-to-use : No need to heat, dilute or add reducing agents before use.



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